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alk4 ca cat 27223 dna plasmids  (Addgene inc)


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    Structured Review

    Addgene inc alk4 ca cat 27223 dna plasmids
    Chromosome squashes of <t>ALK4</t> KO cells showing examples of fluorescently labeled (DAPI) chromosomes prepared after blockage in metaphase. The histograms show the number of times a particular number of chromosome pairs were found (n-77). The same data is binned as 5 (B) or 1 (C) chromosome/cell.
    Alk4 Ca Cat 27223 Dna Plasmids, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/alk4+ca+cat+27223+dna+plasmids/bio_rxiv__64898__2025__12__13__694153-310-6-15?v=Addgene+inc
    Average 93 stars, based on 2 article reviews
    alk4 ca cat 27223 dna plasmids - by Bioz Stars, 2026-08
    93/100 stars

    Images

    1) Product Images from "A Model for the Diversity Explosion Fundamental to Metastasis"

    Article Title: A Model for the Diversity Explosion Fundamental to Metastasis

    Journal: bioRxiv

    doi: 10.64898/2025.12.13.694153

    Chromosome squashes of ALK4 KO cells showing examples of fluorescently labeled (DAPI) chromosomes prepared after blockage in metaphase. The histograms show the number of times a particular number of chromosome pairs were found (n-77). The same data is binned as 5 (B) or 1 (C) chromosome/cell.
    Figure Legend Snippet: Chromosome squashes of ALK4 KO cells showing examples of fluorescently labeled (DAPI) chromosomes prepared after blockage in metaphase. The histograms show the number of times a particular number of chromosome pairs were found (n-77). The same data is binned as 5 (B) or 1 (C) chromosome/cell.

    Techniques Used: Labeling

    When entering mitosis cells have maximum volumes, have rounded up, slightly detached from the substrate and therefore can be modeled as spherical. Cells cultures of equal confluence (60-80%) were imaged at 4 min intervals for 24 hr. Videos of cell cultures were observed both in forward and reverse to determine the last frame before cytokinesis. The diameter of each was then measured using ImageJ and volumes calculated as 4/3p(d/2) 3 . Consistent with the distributions of nuclear sizes, we find that the KO cell population were skewed toward the very small. Both normally passaged KO cultures and those regrown from cells gently rocked off from that culture showed very similar distributions. N=50 (EV), 144 (ALK4 KO), 86 (Rocked Off). A-C: Data plotted for individual cultures, D: Same data plotted together.
    Figure Legend Snippet: When entering mitosis cells have maximum volumes, have rounded up, slightly detached from the substrate and therefore can be modeled as spherical. Cells cultures of equal confluence (60-80%) were imaged at 4 min intervals for 24 hr. Videos of cell cultures were observed both in forward and reverse to determine the last frame before cytokinesis. The diameter of each was then measured using ImageJ and volumes calculated as 4/3p(d/2) 3 . Consistent with the distributions of nuclear sizes, we find that the KO cell population were skewed toward the very small. Both normally passaged KO cultures and those regrown from cells gently rocked off from that culture showed very similar distributions. N=50 (EV), 144 (ALK4 KO), 86 (Rocked Off). A-C: Data plotted for individual cultures, D: Same data plotted together.

    Techniques Used:



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    93
    Addgene inc alk4 ca cat 27223 dna plasmids
    Chromosome squashes of <t>ALK4</t> KO cells showing examples of fluorescently labeled (DAPI) chromosomes prepared after blockage in metaphase. The histograms show the number of times a particular number of chromosome pairs were found (n-77). The same data is binned as 5 (B) or 1 (C) chromosome/cell.
    Alk4 Ca Cat 27223 Dna Plasmids, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/alk4+ca+cat+27223+dna+plasmids/bio_rxiv__64898__2025__12__13__694153-310-6-15?v=Addgene+inc
    Average 93 stars, based on 1 article reviews
    alk4 ca cat 27223 dna plasmids - by Bioz Stars, 2026-08
    93/100 stars
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    Image Search Results


    Chromosome squashes of ALK4 KO cells showing examples of fluorescently labeled (DAPI) chromosomes prepared after blockage in metaphase. The histograms show the number of times a particular number of chromosome pairs were found (n-77). The same data is binned as 5 (B) or 1 (C) chromosome/cell.

    Journal: bioRxiv

    Article Title: A Model for the Diversity Explosion Fundamental to Metastasis

    doi: 10.64898/2025.12.13.694153

    Figure Lengend Snippet: Chromosome squashes of ALK4 KO cells showing examples of fluorescently labeled (DAPI) chromosomes prepared after blockage in metaphase. The histograms show the number of times a particular number of chromosome pairs were found (n-77). The same data is binned as 5 (B) or 1 (C) chromosome/cell.

    Article Snippet: The ALK4 WT (Cat# 80879) and ALK4 CA (Cat# 27223) DNA plasmids were purchased from Addgene.

    Techniques: Labeling

    When entering mitosis cells have maximum volumes, have rounded up, slightly detached from the substrate and therefore can be modeled as spherical. Cells cultures of equal confluence (60-80%) were imaged at 4 min intervals for 24 hr. Videos of cell cultures were observed both in forward and reverse to determine the last frame before cytokinesis. The diameter of each was then measured using ImageJ and volumes calculated as 4/3p(d/2) 3 . Consistent with the distributions of nuclear sizes, we find that the KO cell population were skewed toward the very small. Both normally passaged KO cultures and those regrown from cells gently rocked off from that culture showed very similar distributions. N=50 (EV), 144 (ALK4 KO), 86 (Rocked Off). A-C: Data plotted for individual cultures, D: Same data plotted together.

    Journal: bioRxiv

    Article Title: A Model for the Diversity Explosion Fundamental to Metastasis

    doi: 10.64898/2025.12.13.694153

    Figure Lengend Snippet: When entering mitosis cells have maximum volumes, have rounded up, slightly detached from the substrate and therefore can be modeled as spherical. Cells cultures of equal confluence (60-80%) were imaged at 4 min intervals for 24 hr. Videos of cell cultures were observed both in forward and reverse to determine the last frame before cytokinesis. The diameter of each was then measured using ImageJ and volumes calculated as 4/3p(d/2) 3 . Consistent with the distributions of nuclear sizes, we find that the KO cell population were skewed toward the very small. Both normally passaged KO cultures and those regrown from cells gently rocked off from that culture showed very similar distributions. N=50 (EV), 144 (ALK4 KO), 86 (Rocked Off). A-C: Data plotted for individual cultures, D: Same data plotted together.

    Article Snippet: The ALK4 WT (Cat# 80879) and ALK4 CA (Cat# 27223) DNA plasmids were purchased from Addgene.

    Techniques: